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Article

A Radiolabeling-Free, qPCR-Based Method for Locus-Specific Pseudouridine Detection.

Angewandte Chemie (International ed. in English) - 20 Nov 2017

Lei Zhixin, Yi Chengqi

Abstract excerpt

Pseudouridine (Ψ) is the most abundant post-transcriptional RNA modification. Methods have been developed for locus-specific Ψ detection; however, they often involve radiolabeling of RNA, require advanced experimental skills, and can be time-consuming. Herein we report a radiolabeling-free, qPCR-based method to rapidly detect locus-specific Ψ. Pseudouridine residues were labeled chemically, and the resulting...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 28960747
DOI: 10.1002/anie.201708276

Genes

PSME2
PUS7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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