Towards CRISPR/Cas9-based gene drive in the diamondback moth <i>Plutella xylostella</i>

Abstract excerpt

Promising to provide powerful genetic control tools, gene drives have been constructed in multiple dipterans, yeast and mice, for the purposes of population elimination or modification. However, it remains unclear whether these techniques can be applied to lepidopterans. Here, we used endogenous regulatory elements to drive Cas9 and sgRNA expression in the diamondback moth, ( Plutella xylostella ), and test the f...

Topics

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• CRISPR-Cas Systems
• Gene Editing
• Gene Knockout Techniques
• Gene Order
• Gene Targeting
• Genotyping Techniques
• RNA, Guide, CRISPR-Cas Systems
• Zygote

Identifiers and source

Literature Corpus work

5bebda04-2e1f-56cf-9a3e-279887b2cd84

DOI

10.1101/2021.10.05.462963