Article

Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic

2022-04-19

Abstract excerpt

<title>Abstract</title> <p>Type-III CRISPR-Cas systems have recently been adopted for sequence-specific detection of SARS-CoV-2. Here, we make two major advances that simultaneously limit sample handling and significantly enhance the sensitivity of SARS-CoV-2 RNA detection directly from patient samples. First, we repurpose the type III-A CRISPR complex from Thermus thermophilus (TtCsm) for programmable capture an...

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Literature Corpus work: 59d95cef-2543-56a3-8315-9f0b1184ad1e
DOI: 10.21203/rs.3.rs-1466718/v1

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