Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic

Abstract excerpt

Abstract Type-III CRISPR-Cas systems have recently been adopted for sequence-specific detection of SARS-CoV-2. Here, we repurpose the type III-A CRISPR complex from Thermus thermophilus (TtCsm) for programmable capture and concentration of specific RNAs from complex mixtures. The target bound TtCsm complex generates two cyclic oligoadenylates (i.e., cA 3 and cA 4 ) that allosterically activate ancillary...

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