Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells

Abstract excerpt

Type V CRISPR–Cas12a systems are an attractive Cas9-alternative nuclease platform for specific genome-editing applications. However, previous studies demonstrate that there is a gap in overall activity between Cas12a and Cas9 in primary cells. Here we describe optimization to the nuclear localization signal (NLS) composition and architecture of Cas12a to facilitate highly efficient targeted mutagenesis in human...

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