Article

A cellular stress response induced by the CRISPR/dCas9 activation system is not heritable through cell divisions

2019-12-23

Abstract excerpt

<h4>ABSTRACT</h4> The CRISPR/Cas9 system can be modified to perform ‘epigenetic editing’ by utilizing the catalytically-inactive (dead) Cas9 (dCas9) to recruit regulatory proteins to specific genomic locations. In prior studies, epigenetic editing with multimers of the transactivator VP16 and guide RNAs (gRNAs) was found to cause adverse cellular responses. These side effects may confound studies inducing new cel...

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Identifiers and source

Literature Corpus work: 50437237-3f8e-54f9-bd67-e0c18e1ffadd
DOI: 10.1101/2019.12.23.887224

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