Article

Single-cell Rapid Capture Hybridization sequencing (scRaCH-seq) to reliably detect isoform usage and coding mutations in targeted genes at a single-cell level

2024-01-30

Abstract excerpt

Single-cell long-read sequencing has transformed our understanding of isoform usage and the mutation heterogeneity between cells. Despite unbiased in-depth analysis, the low sequencing throughput often results in insufficient read coverage thereby limiting our ability to perform mutation calling for specific genes. Here, we developed a s ingle- c ell Ra pid Capture H ybridization seq uencing (scRaCH-seq) meth...

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Literature Corpus work: 49ca0472-9f5c-54ae-9948-a39bbc153717
DOI: 10.1101/2024.01.30.577942

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