Article
Single-cell Rapid Capture Hybridization sequencing reliably detects isoform usage and coding mutations in targeted genes.
Genome research - 14 Apr 2025
Peng Hongke, Jabbari Jafar S, Tian Luyi, Wang Changqing, You Yupei, Chua Chong Chyn, Anstee Natasha S, Amin Noorul, Wei Andrew H, Davidson Nadia M, Roberts Andrew W, Huang David C S, Ritchie Matthew E, Thijssen Rachel
Abstract excerpt
Single-cell long-read sequencing has transformed our understanding of isoform usage and the mutation heterogeneity between cells. Despite unbiased in-depth analysis, the low sequencing throughput often results in insufficient read coverage, thereby limiting our ability to perform mutation calling for specific genes. Here, we developed a single-cell Rapid Capture Hybridization sequencing (scRaCH-seq) method that...
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