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Article

Quantitation of HIV-1 DNA with a sensitive TaqMan assay that has broad subtype specificity.

Journal of virological methods - 1 Jan 2013

van der Sluis Renée M, van Montfort Thijs, Centlivre Mireille, Schopman Nick C T, Cornelissen Marion, Sanders Rogier W, Berkhout Ben, Jeeninga Rienk E, Paxton William A, Pollakis Georgios

Abstract excerpt

The increasing diversity of HIV-1 isolates makes virus quantitation challenging, especially when diverse isolates co-circulate in a geographical area. Measuring the HIV-1 DNA levels in cells has become a valuable practical tool for fundamental and clinical research. A quantitative HIV-1 DNA assay was developed based on TaqMan(®) technology. Primers that target the highly conserved LTR region were designed to...

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Share to DNA, Viral
Share to HIV-1

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to HIV Long Terminal Repeat

Share to In Situ Hybridization, Fluorescence

Share to RNA, Viral

Share to Sensitivity and Specificity

Share to T-Lymphocytes

Share to Taq Polymerase

Source

Identifiers

PMID: 23059551
DOI: 10.1016/j.jviromet.2012.09.019

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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