Article
In-house HIV-1 RNA real-time RT-PCR assays: principle, available tests and usefulness in developing countries
1 Sept 2008
Abstract excerpt
The principle of currently available licensed HIV-1 RNA assays is based on real-time technologies that continuously monitor the fluorescence emitted by the amplification products. Besides these assays, in-house quantitative (q) real-time reverse transcription (RT)-PCR (RT-qPCR) tests have been developed and evaluated particularly in developing countries, for two main reasons. First, affordable and generalized...