Article

Features affecting Cas9-Induced Editing Efficiency and Patterns in Tomato: Evidence from a Large CRISPR Dataset

2026-01-07

Abstract excerpt

CRISPR/Cas9 is a cornerstone of genome editing, yet the determinants of editing efficiency and DNA Double Strand Break (DSB) repair outcomes remain poorly understood, particularly in plants. To address this gap, we generated a dataset of 420 sgRNAs targeting promoters, exons, and introns of 137 genes in tomato protoplasts and quantified editing efficiencies together with ATAC-seq–derived chromatin accessibility an...

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Literature Corpus work: 3b599c6b-6439-52c1-b980-f00d4c277b41
DOI: 10.64898/2026.01.06.696182

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