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Article

Quantifying allele-specific CRISPR editing activity with CRISPECTOR2.0.

Nucleic acids research - 9 Sept 2024

Assa Guy, Kalter Nechama, Rosenberg Michael, Beck Avigail, Markovich Oshry, Gontmakher Tanya, Hendel Ayal, Yakhini Zohar

Abstract excerpt

Off-target effects present a significant impediment to the safe and efficient use of CRISPR-Cas genome editing. Since off-target activity is influenced by the genomic sequence, the presence of sequence variants leads to varying on- and off-target profiles among different alleles or individuals. However, a reliable tool that quantifies genome editing activity in an allelic context is not available. Here, we...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39077930
DOI: 10.1093/nar/gkae651
PMCID: PMC11381363

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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