Article

Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live Mice

2023-12-10

Abstract excerpt

Functional analysis of non-coding variants associated with human congenital disorders remains challenging due to the lack of efficient in vivo models. Here we introduce dual-enSERT, a robust Cas9-based two-color fluorescent reporter system which enables rapid, quantitative comparison of enhancer allele activities in live mice of any genetic background. We use this new technology to examine and measure the gain- a...

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Literature Corpus work: 395965ba-8d33-52e3-b055-3d1e19d4eef8
DOI: 10.1101/2023.12.10.570890

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