Article
Rapid and quantitative functional interrogation of human enhancer variant activity in live mice.
Nature communications - 6 Jan 2025
Hollingsworth Ethan W, Liu Taryn A, Alcantara Joshua A, Chen Cindy X, Jacinto Sandra H, Kvon Evgeny Z
Abstract excerpt
Functional analysis of non-coding variants associated with congenital disorders remains challenging due to the lack of efficient in vivo models. Here we introduce dual-enSERT, a robust Cas9-based two-color fluorescent reporter system which enables rapid, quantitative comparison of enhancer allele activities in live mice in less than two weeks. We use this technology to examine and measure the gain- and...
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