Article

RNA-guided endonuclease-driven mutagenesis in tobacco followed by efficient fixation of mutated sequences in doubled haploid plants

2016-03-03

Abstract excerpt

<h4>Background</h4> Customizable endonucleases are providing an effective tool for genome engineering. The resulting primary transgenic individuals are typically heterozygous and/or chimeric with respect to any mutations induced. To generate genetically fixed mutants, they are conventionally allowed to self-pollinate, a procedure which segregates individuals into mutant heterozygotes/homozygotes and wild types. T...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 38a05f7c-344f-5b5f-aed0-976dfa6420f0
DOI: 10.1101/042291

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

RNA-guided endonuclease-driven mutagenesis in tobacco followed by efficient fixation of mutated sequences in doubled haploid plantsDOI 10.1101/042291Read

Impact of temperature and time on DNA-free Cas9-ribonucleoprotein mediated gene editing in wheat protoplasts and immature embryosSemantic proximity Read

Efficient allelic-drive in Drosophila.Semantic proximity Read

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles.Semantic proximity Read

Viral delivery of an RNA-guided genome editor for transgene-free germline editing in <i>Arabidopsis</i>Semantic proximity Read

Viral delivery of recombinases to activate heritable genetic switches in plantsSemantic proximity Read

Cleave and Rescue gamete killers create conditions for gene drive in plants.Semantic proximity Read

Impacts of RNA Mobility Signals on Virus Induced Somatic and Germline Gene EditingSemantic proximity Read

Nonhomologous end joining-mediated gene replacement in plant cells.Semantic proximity Read

Heritable base-editing in Arabidopsis using RNA viral vectors.Semantic proximity Read

Induction, rapid fixation and retention of mutations in vegetatively propagated banana.Semantic proximity Read

Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.Semantic proximity Read

Directed microspore-specific recombination of transgenic alleles to prevent pollen-mediated transmission of transgenes.Semantic proximity Read