Article

Tailoring a CRISPR/Cas-based Epigenome Editor for Programmable Chromatin Acylation and Decreased Cytotoxicity

2024-09-22

Abstract excerpt

Engineering histone acylation states can inform mechanistic epigenetics and catalyze therapeutic epigenome editing opportunities. Here, we developed engineered lysine acyltransferases that enable the programmable deposition of acetylation and longer-chain acylations. We show that targeting an engineered lysine crotonyltransferase results in weak levels of endogenous enhancer activation yet retains potency when tar...

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Identifiers and source

Literature Corpus work: 384f3523-fde8-52aa-b333-e488a0b9a855
DOI: 10.1101/2024.09.22.611000

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