Article

Mapping Structural Constraints and Adaptive Potential in a Capsule-Degrading Phage Tailspike Protein

2025-10-06

Abstract excerpt

Bacteriophage tailspike proteins (TSPs) degrade bacterial capsules to enable infection, yet the molecular determinants of their function and host range remain unclear. We applied deep mutational scanning (DMS) to the endosialidase TSP of Escherichia coli K1 phage K1F, generating 22,365 single-amino-acid variants using an enhanced ORACLE phage engineering platform. Functional scores revealed the TSP is structurall...

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Literature Corpus work: 372b5c7b-2904-518a-ab71-6dc30a9916cb
DOI: 10.1101/2025.10.06.680778

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