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Article

Mapping structural constraints and adaptive potential in a capsule-degrading phage tailspike protein.

Science advances - 11 Sept 2026

Evert Sarah, Huss Phil, Kuppa Baskaran Dinesh Kumar, Anantharaman Karthik, Raman Srivatsan

Abstract excerpt

Bacteriophage tailspike proteins (TSPs) degrade bacterial capsules to enable infection, yet the molecular determinants of their function and host range remain unclear. We applied deep mutational scanning (DMS) to the endosialidase TSP of Escherichia coli K1 phage K1F, generating 22,365 single-amino acid variants using an enhanced ORACLE phage engineering platform. Functional scores revealed that the TSP is...

Read the complete abstract on PubMed

Topics

Viral Tail Proteins
Bacterial Capsules
Mutation
Escherichia coli
Models, Molecular
Catalytic Domain

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Bacteriophages

Host Specificity

Binding Sites

Glycoside Hydrolases

Source

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Identifiers

PMID: 42726877
DOI: 10.1126/sciadv.aed3641
PMCID: PMC13564897

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 24 Oct 2026.

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