Article

INTERACT: Interactome Network Targeting via Enzyme Reactivity and Activity-based Chemoproteomic Tools

2025-11-04

Abstract excerpt

This chapter details an activity-based chemoproteomic methodology, INTERACT (Interactome Network Targeting via Enzyme Reactivity and Activity-based Chemoproteomic Tools), for the functional interrogation of host-pathogen interactions (interactome) in a physiologically relevant context. The exemplar protocol utilises a small, cell-permeable fluorophosphonate probe to covalently label active serine hydrolases simult...

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Literature Corpus work: 34fb8edd-2e25-58e2-a121-a2098ae5a32b
DOI: 10.1101/2025.11.03.686322

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