Article

Paradoxical effects of Zim3, a CRISPRi effector, on human iPSC-cardiomyocyte electrophysiology

2023-06-10

Abstract excerpt

We show that Zim3, when used as Zim3-KRAB-dCas9 effector in interference CRISPR, without any guide RNAs, paradoxically upregulates key cardiac ion channel genes in human induced pluripotent stem-cell-derived cardiomyocytes, iPSC-CMs, responsible for healthy resting membrane potential, repolarization of the action potential and electrical transmission of signals. These were found to yield expected functional enhanc...

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Literature Corpus work: 33ee3309-28b1-5d3f-9c3a-1f99c9a2bb14
DOI: 10.1101/2023.06.10.544468

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