Cell-Free Synthetic Biosensors Based on CRISPR/Cas Mediated Cascade Signal Amplification for Precise RNA Detection

Abstract excerpt

<title>Abstract</title> <p>Synthetic biology has been harnessed to create new diagnostic technologies. However, most synthetic biosensors involve error-prone amplification steps and limitations of accuracy in RNA detection. Here, we report a cell-free synthetic biosensing platform, termed as SHARK (<underline>S</underline>ynthetic Enzyme S<underline>h</underline>ift RNA Signal <underline>A</underline>mplifier <un...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Genotyping Techniques
• Leukemia, Myeloid, Acute
• Nucleic Acid Amplification Techniques
• Pancreatic Neoplasms
• Point-of-Care Systems

Identifiers and source

Literature Corpus work

32fa8aff-6c13-54fa-b98c-c1e2a12adceb

DOI

10.21203/rs.3.rs-358218/v1