Article

Spatial Relationships Matter: Kinesin-1 Molecular Motors Transport Liposome Cargo Through 3D Microtubule Intersections <i>In Vitro</i>

2023-12-02

Abstract excerpt

Kinesin-1 ensembles maneuver vesicular cargoes through intersections in the 3-dimensional (3D) intracellular microtubule (MT) network. To characterize directional outcomes (straight, turn, terminate) at MT intersections, we challenge 350 nm fluid-like liposomes transported by ∼10 constitutively active, truncated kinesin-1 KIF5B (K543) with perpendicular 2-dimensional (2D) and 3D intersections in vitro . Liposomes...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 2f039071-4569-53dd-8406-7f1b6ef0d01b
DOI: 10.1101/2023.12.01.569616

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Spatial Relationships Matter: Kinesin-1 Molecular Motors Transport Liposome Cargo Through 3D Microtubule Intersections <i>In Vitro</i>DOI 10.1101/2023.12.01.569616Read

Tau directs intracellular trafficking by regulating the forces exerted by kinesin and dynein teamsSemantic proximity Read

Intrinsically disordered domain of kinesin-3 Kif14 enables unique functional diversitySemantic proximity Read

Direct observation of intermediate states during the stepping motion of kinesin-1.Semantic proximity Read

KIF1A is kinetically tuned to be a super-engaging motor under hindering loadsSemantic proximity Read

Single-particle fluorescence tracking combined with TrackMate assay reveals highly heterogeneous and discontinuous lysosomal transport in freely orientated axonsSemantic proximity Read

The sets of kinesins and dynein transporting endocytic cargoes determine the effect of tau on their motilitySemantic proximity Read

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1Semantic proximity Read

KIF1A is kinetically tuned to be a superengaging motor under hindering loads.Semantic proximity Read

Obstacles on the microtubule reduce the processivity of Kinesin-1 in a minimal in vitro system and in cell extract.Semantic proximity Read

Branching, crosslinking and decentralization of microtubules accelerates intracellular assemblySemantic proximity Read

Human kinesin-5 KIF11 drives the helical motion of anti-parallel and parallel microtubules around each otherSemantic proximity Read

Mitochondrial mechanics nucleates axonal jamming and swellingSemantic proximity Read