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Article

Directed Evolution of Heterologous tRNAs Leads to Reduced Dependence on Post-transcriptional Modifications.

ACS synthetic biology - 18 May 2018

Baldridge Kevin C, Jora Manasses, Maranhao Andre C, Quick Matthew M, Addepalli Balasubrahmanyam, Brodbelt Jennifer S, Ellington Andrew D, Limbach Patrick A, Contreras Lydia M

Abstract excerpt

Heterologous tRNA:aminoacyl tRNA synthetase pairs are often employed for noncanonical amino acid incorporation in the quest for an expanded genetic code. In this work, we investigated one possible mechanism by which directed evolution can improve orthogonal behavior for a suite of Methanocaldococcus jannaschii ( Mj) tRNATyr-derived amber suppressor tRNAs. Northern blotting demonstrated that reduced expression of...

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Identifiers

PMID: 29694026
DOI: 10.1021/acssynbio.7b00421

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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