Article
Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing in Human Cells.
Molecular therapy : the journal of the American Society of Gene Therapy - 1 Feb 2017
Havlicek Steven, Shen Yang, Alpagu Yunus, Bruntraeger Michaela B, Zufir Nurdiana B M, Phuah Zhi Yi, Fu Zhiyan, Dunn Norris R, Stanton Lawrence W
Abstract excerpt
Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 enables us to generate targeted sequence changes in the genomes of cells and organisms. However, off-target effects have been a persistent problem hampering the development of therapeutics based on CRISPR/Cas9 and potentially confounding research results. Efforts to improve Cas9 specificity, like the development of RNA-guided FokI-nucleases (RFNs),...
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