Article

A multi-species toolkit of <i>TOP2</i> hypercleavage mutants for studying topoisomerase II–mediated DNA damage

2026-05-11

Abstract excerpt

DNA topoisomerase II (TOP2) generates transient DNA double-strand breaks that are trapped as TOP2-DNA covalent complexes (TOP2cc) by antibiotic and chemotherapy drugs. Here, we characterize tools for study of cellular responses to TOP2cc, exploiting a Saccharomyces cerevisiae TOP2 mutant ( TOP2-F1025Y,R1128G ) that generates spontaneous and inhibitor-induced covalent complexes at elevated frequencies. This Top2-...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 2cf5f361-b41c-59f3-aca7-7b58ab9d9da1
DOI: 10.64898/2026.05.09.723998

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A multi-species toolkit of <i>TOP2</i> hypercleavage mutants for studying topoisomerase II–mediated DNA damageDOI 10.64898/2026.05.09.723998Read

Trapped Topoisomerase II initiates formation of <i>de novo</i> duplications <i>via</i> the nonhomologous end-joining pathway in yeastSemantic proximity Read

Recurrent mutations in topoisomerase IIα cause a previously undescribed mutator phenotype in human cancers.Semantic proximity Read

Isolation and characterization of mAMSA-hypersensitive mutants. Cytotoxicity of Top2 covalent complexes containing DNA single strand breaks.Semantic proximity Read

Trapped topoisomerase II initiates formation of de novo duplications via the nonhomologous end-joining pathway in yeastSemantic proximity Read

A Yeast-Based Screening System for Differential Identification of Poison Inhibitors and Catalytic Inhibitors of Human Topoisomerase ISemantic proximity Read

Topoisomerase 3B (TOP3B) DNA and RNA Cleavage Complexes and Pathway to Repair TOP3B-linked RNA and DNA BreaksSemantic proximity Read

TDP1-independent pathways in the process and repair of TOP1-induced DNA damageSemantic proximity Read

SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.Semantic proximity Read

GCNA interacts with Spartan and Topoisomerase II to regulate genome stabilitySemantic proximity Read

Mutations in topoisomerase IIβ result in a B cell immunodeficiencySemantic proximity Read

Mutability and mutational spectrum of chromosome transmission fidelity genes.Semantic proximity Read

Mutational signatures of DNA mismatch repair deficiency in <i>C. elegans</i> and human cancersSemantic proximity Read