Article

A genetically-encoded fluorescent biosensor for visualization of acetyl-CoA in live cells

2024-01-01

Abstract excerpt

Acetyl-coenzyme A is a central metabolite that participates in many cellular pathways. Evidence suggests that acetyl-CoA production and consumption are highly compartmentalized in mammalian cells. Yet methods to measure acetyl-CoA in living cells are lacking. In this work, we engineer an acetyl-CoA biosensor from the bacterial protein PanZ and circularly permuted green fluorescent protein (cpGFP). We biochemically...

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Literature Corpus work: 2ac6e842-1b61-5d4f-8464-a9503e190931
DOI: 10.1101/2023.12.31.573774

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