Article

A systems view of spliceosomal assembly and branchpoints with iCLIP

2018-06-22

Abstract excerpt

Studies of spliceosomal interactions are challenging due to their dynamic nature. Here we employed spliceosome iCLIP, which immunoprecipitates SmB along with snRNPs and auxiliary RNA binding proteins (RBPs), to map human spliceosome engagement with snRNAs and pre-mRNAs. This identified over 50,000 branchpoints (BPs) that have canonical sequence and structural features. Moreover, it revealed 7 binding peaks around...

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Literature Corpus work: 26d99a61-646f-5e46-a6b3-00e1203f5f05
DOI: 10.1101/353599

Open publication

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