Article

De Novo Negatively Charged Binders Targeting MMLVRT Nucleic Acid Binding Sites Overcome Stability-Activity Trade-offs

2025-03-17

Abstract excerpt

The limited thermostability and storage-induced inactivation of Moloney murine leukemia virus reverse transcriptase (MMLV RT) have constrained its applications. In this study, a high-stability mutant, MMLV RT-SV, was generated through a multi-site mutagenesis strategy targeting the nucleic acid-binding pocket. Through de novo protein design, three highly negatively charged binders with affinities of 45 nM, 362 nM,...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 20c7c191-049a-5b19-a9ae-3eddcb12f978
DOI: 10.1101/2025.03.15.643416

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

De Novo Negatively Charged Binders Targeting MMLVRT Nucleic Acid Binding Sites Overcome Stability-Activity Trade-offsDOI 10.1101/2025.03.15.643416Read

Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis.Semantic proximity Read

Increasing the melting temperature of VHH with the in silico free energy score.Semantic proximity Read

Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.Semantic proximity Read

Rational Design of Inhibitors of HIV-1 TAR RNA through the Stabilisation of Electrostatic “Hot Spots”Semantic proximity Read

Lock, Protect, and Bind: In Vitro Selection of LNA-modified Aptamers Using a Mutant T7 RNA PolymeraseSemantic proximity Read

A rapid, ensemble and free energy based method for engineering protein stabilities.Semantic proximity Read

Harnessing Fluorine-Sulfur Contacts and Multipolar Interactions for the Design of p53 Mutant Y220C Rescue Drugs.Semantic proximity Read

Crystal engineering of HIV-1 reverse transcriptase for structure-based drug designSemantic proximity Read

Engineering UvsY solubility through local hydrophobic cluster disruption enables enhanced recombination mediator activity.Semantic proximity Read

Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold LibrarySemantic proximity Read

Single-residue engineering of Klenow fragment enables broad acceptance of chemically modified nucleotides.Semantic proximity Read

Identification of the structural basis of thermal lability of a virus provides a rationale for improved vaccines.Semantic proximity Read