Lock, Protect, and Bind: In Vitro Selection of LNA-modified Aptamers Using a Mutant T7 RNA Polymerase

Abstract excerpt

RNA therapeutics are powerful tools for gene modulation and targeted therapies, but their clinical application is hindered by nuclease degradation and immunogenicity. Incorporating chemical modifications, like locked nucleic acids (LNAs), can enhance nuclease resistance, targeting properties, and thermal stability. Traditionally, LNA incorporation has relied on solid-phase synthesis of short RNAs. Engineered polym...

Topics

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• Aptamers, Nucleotide
• Base Sequence
• DNA-Directed DNA Polymerase
• Mutation
• Oligonucleotides
• RNA and protein synthesis mechanisms
• RNA Interference and Gene Delivery
• Viral Infections and Immunology Research

Identifiers and source

Literature Corpus work

898285ed-f711-5a47-9478-1a30928bd5a3

DOI

10.1101/2025.04.09.647959