Engineering UvsY solubility through local hydrophobic cluster disruption enables enhanced recombination mediator activity.

Abstract excerpt

The recombination mediator UvsY plays a critical role in recombinase polymerase amplification but is intrinsically limited by poor solubility and aggregation at high protein concentrations. Here, we present a systematic strategy to improve UvsY solubility and functional performance by integrating consensus mutagenesis with virtual saturation mutagenesis. A single substitution, F73P, was identified as a core...

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• Hydrophobic and Hydrophilic Interactions
• Molecular Dynamics Simulation
• Mutation
• Protein Engineering
• Recombination, Genetic