Back to search

Article

High-efficiency Non-viral Transfection of Human Primary T Lymphocytes

2020-05-28

Abstract excerpt

The development and optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells is a major challenge in clinical immunotherapy researches. According to the low transfection efficiency of conventional methods in human primary T cells, there is an effort in order to increase the transfection rate in these cells. Protamine is an FDA-approved compound with a documented...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
1fff0cec-bcfd-5465-a1f0-923a0796eab1
DOI
10.20944/preprints202005.0456.v1
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
High-efficiency Non-viral Transfection of Human Primary T LymphocytesDOI 10.20944/preprints202005.0456.v1
Optimized DNA electroporation for primary human T cell engineeringSemantic proximity
Viable and efficient electroporation-based genetic manipulation of unstimulated human T cellsSemantic proximity
Nonviral gene delivery to T cells with Lipofectamine LTXSemantic proximity
Sequentially conquering transfection obstacles of primary human T cells via non-classical lipid nanoparticles delivering reformative transposon systemSemantic proximity
Optimization of electroporation and other non‐viral gene delivery strategies for T cellsSemantic proximity
Non-viral transfection technologies for next-generation therapeutic T cell engineeringSemantic proximity
Optimizing Transfection Efficiency in CAR-T Cell Manufacturing through Multiple Administrations of Lipid-Based NanoparticlesSemantic proximity
Calcium enhances polyplex-mediated transfection efficiency of plasmid DNA in Jurkat cellsSemantic proximity
Systematic optimization of siRNA productive uptake into resting and activated T cells <i>ex vivo</i>Semantic proximity
Human T Lymphocyte Genetic Modification with Naked DNASemantic proximity
Optimized conditions for gene transduction into primary immune cells using viral vectorsSemantic proximity
A solution for highly efficient electroporation of primary cytotoxic T lymphocytesSemantic proximity
Select a neighboring publication to make it the new centre.