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Calcium enhances polyplex-mediated transfection efficiency of plasmid DNA in Jurkat cells

1 Jan 2020

Abstract excerpt

Jurkat, an immortalized cell line derived from human leukemic T lymphocytes, has been employed as an excellent surrogate model of human primary T-cells for the advancement of T-cell biology and their applications in medicine. However, presumably due to its T-cell origin, Jurkat cells are very difficult to transfect. Thus, for the genetic modification of Jurkat cells, expensive and time-consuming viral vectors are...

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PMID: 32489110
DOI: 10.1080/10717544.2020.1770371

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