Article

Probing recombinant AAV capsid integrity and genome release under thermal stress by single-molecule interferometric scattering microscopy

2024-03-07

Abstract excerpt

Adeno-associated viruses (AAVs) are gaining traction as delivery vehicles for gene therapy although the molecular understanding of AAV-transgene release is still limited. Typically, the process of viral uncoating is investigated ( in vitro ) through thermal stress, revealing capsid disintegration at elevated temperatures. Here, we used single-molecule interferometric scattering microscopy to assess the (in)stabil...

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Literature Corpus work: 1e783a20-64d3-57c4-96d5-3e6c41c2cf17
DOI: 10.1101/2024.03.07.583968

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