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Recombinant AAV genome size effect on viral vector production, purification, and thermostability

17 Jan 2024

Abstract excerpt

Adeno-associated virus (AAV) has shown great promise as a viral vector for gene therapy in clinical applications. The present work studied the effect of genome size on AAV production, purification, and thermostability by producing AAV2-GFP using suspension-adapted HEK293 cells via triple transfection using AAV plasmids containing the same GFP transgene with DNA stuffers for variable-size AAV genomes consisting of...

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PMID: 38327806
DOI: 10.1016/j.omtm.2024.101188

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