Article
rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations.
International journal of molecular sciences - 14 Nov 2019
Feiner Rebecca C, Teschner Julian, Teschner Kathrin E, Radukic Marco T, Baumann Tobias, Hagen Sven, Hannappel Yvonne, Biere Niklas, Anselmetti Dario, Arndt Katja M, Müller Kristian M
Abstract excerpt
Recombinant adeno-associated viruses (rAAV) provide outstanding options for customization and superior capabilities for gene therapy. To access their full potential, facile genetic manipulation is pivotal, including capsid loop modifications. Therefore, we assessed capsid tolerance to modifications of the structural VP proteins in terms of stability and plasticity. Flexible glycine-serine linkers of increasing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.