Article

Handling of Spurious Sequences Affects the Outcome of High-Throughput 16S rRNA Gene Amplicon Profiling

2020-12-02

Abstract excerpt

<title>Abstract</title> <p><bold>Background: </bold>16S rRNA gene amplicon sequencing<bold> </bold>is a very popular approach for studying microbiomes. However, varying standards exist for sample and data processing and some basic concepts, such as the occurrence of spurious sequences, have not been investigated in a comprehensive manner. <bold>Methods: </bold>Using defined communities of bacteria <italic>in vitr...

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Literature Corpus work: 1e212d73-4a5e-555f-8b14-508f11383790
DOI: 10.21203/rs.3.rs-117421/v1

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