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Article

Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing.

mSphere - 24 Feb 2021

Abellan-Schneyder Isabel, Matchado Monica S, Reitmeier Sandra, Sommer Alina, Sewald Zeno, Baumbach Jan, List Markus, Neuhaus Klaus

Abstract excerpt

Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies investigating microbiomes. However, comparative studies on differences in procedures are scarce. We sequenced human stool samples and mock communities with increasing complexity using a variety of commonly used protocols. Short amplicons targeting different variable regions (V-regions) or ranges thereof (V1-V2, V1-V3, V3-V4, V4,...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 33627512
DOI: 10.1128/mSphere.01202-20
PMCID: PMC8544895

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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