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Article

Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling

29 Jun 2021

Abstract excerpt

16S rRNA gene amplicon sequencing is a popular approach for studying microbiomes. However, some basic concepts have still not been investigated comprehensively. We studied the occurrence of spurious sequences using defined microbial communities based on data either from the literature or generated in three sequencing facilities and analyzed via both operational taxonomic units (OTUs) and amplicon sequence...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 37938227
DOI: 10.1038/s43705-021-00033-z

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