Article

Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling

2020-01-17

Abstract excerpt

<title>Abstract</title> <p>Background: 16S rRNA gene amplicon sequencing is a very popular approach for studying microbiomes. However, varying standards exist for sample and data processing and some basic concepts such as the occurrence of spurious sequences have not been investigated in a comprehensive manner, which was done in the present study. <h4>Methods:</h4> Using defined communities of bacteria in vitro a...

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Literature Corpus work: 3876ff66-8d57-5b62-883f-a2204892f227
DOI: 10.21203/rs.2.21240/v1

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