Article
Microbiome Analysis Using 16S Amplicon Sequencing: From Samples to ASVs.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Amir Amnon
Abstract excerpt
In this chapter, we will present an outline of a typical experimental and bioinformatic workflow for identification of bacterial amplicon sequence variants (ASVs) present in a set of samples. This chapter is written from a bioinformatic point of view; therefore, the specific experimental protocols are not detailed, but rather the impact of various experimental decisions on the downstream analysis is described....
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