Article

Mutational and biophysical robustness in a pre-stabilized monobody

2020-12-14

Abstract excerpt

The fibronectin type III (FN3) monobody domain is a promising non-antibody scaffold which features a less complex architecture than an antibody while maintaining analogous binding loops. We previously developed FN3Con, a hyper-stable monobody derivative with diagnostic and therapeutic potential. Pre-stabilization of the scaffold mitigates the stability-function trade-off commonly associated with evolving a protein...

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Literature Corpus work: 1b263c93-221c-5246-9529-4055d6b34f19
DOI: 10.1101/2020.12.14.422768

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