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Article

Mutational and biophysical robustness in a prestabilized monobody.

The Journal of biological chemistry - 1 Jan 2000

Chandler Peter G, Tan Li Lynn, Porebski Benjamin T, Green James S, Riley Blake T, Broendum Sebastian S, Hoke David E, Falconer Robert J, Munro Trent P, Buckle Malcolm, Jackson Colin J, Buckle Ashley M

Abstract excerpt

The fibronectin type III (FN3) monobody domain is a promising non-antibody scaffold, which features a less complex architecture than an antibody while maintaining analogous binding loops. We previously developed FN3Con, a hyperstable monobody derivative with diagnostic and therapeutic potential. Prestabilization of the scaffold mitigates the stability-function trade-off commonly associated with evolving a protein...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33617878
DOI: 10.1016/j.jbc.2021.100447
PMCID: PMC8010708

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