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Article

The determinants of activity and specificity in actinorhodin type II polyketide ketoreductase.

Chemistry & biology - 24 Oct 2013

Javidpour Pouya, Bruegger Joel, Srithahan Supawadee, Korman Tyler P, Crump Matthew P, Crosby John, Burkart Michael D, Tsai Shiou-Chuan

Abstract excerpt

In the actinorhodin type II polyketide synthase, the first polyketide modification is a regiospecific C9-carbonyl reduction, catalyzed by the ketoreductase (actKR). Our previous studies identified the actKR 94-PGG-96 motif as a determinant of stereospecificity. The molecular basis for reduction regiospecificity is, however, not well understood. In this study, we examined the activities of 20 actKR mutants through...

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Identifiers

PMID: 24035284
DOI: 10.1016/j.chembiol.2013.07.016
PMCID: PMC3855848

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