Article

Nuclei Isolation Protocol for Single-Nucleus RNA Sequencing of Human Stem Cell-Derived Grafts

2025-08-31

Abstract excerpt

<h4>Summary</h4> Single-nucleus RNA sequencing enables high-resolution transcriptomic profiling of brain tissue, facilitating detailed analysis of cell identity in models of neurodegeneration and repair. Here, we describe a protocol for isolating nuclei from long-term human stem cell–derived grafts in the rat brain, incorporating vibratome sectioning, graft dissection, nuclear extraction, and fluorescence-activat...

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Literature Corpus work: 0fb744ea-37ab-574a-ac69-32f423f2d853
DOI: 10.1101/2025.08.27.672369

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