Article

Advancing Nuclei Isolation from Frozen Human Heart for Single-Nucleus RNA Sequencing Applications

2026-03-21

Abstract excerpt

<h4>ABSTRACT</h4> While single-cell RNA sequencing (scRNA-seq) has been the first widely adopted single-cell transcriptomic approach, its reliance on fresh tissue samples has substantially limited its applicability to clinically relevant specimen. Single-nucleus RNA sequencing (snRNA-seq) overcomes this constrain by enabling transcriptomic profiling from frozen material. However, isolating high-quality nuclei fro...

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Literature Corpus work: 5fa6e743-8d4a-5293-aa30-fb3a019ec211
DOI: 10.64898/2026.03.19.712944

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