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Article

Adapting in situ polymerase chain reaction for genotyping of cells in suspension.

Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Oct 1998

Brownie J, Paskins L, Little S, Schwarz M J, Bull J H

Abstract excerpt

An approach is described for in situ polymerase chain reaction (ISPCR) based on cycling primed in situ synthesis (PRINS) conditions defined for alpha-satellite DNA. Using blood cell preparations subjected to limited fixation with paraformaldehyde, ISPCR cycling resulted in a gradual buildup of amplicon at the site of synthesis, as judged by the characteristic presence of paired nuclear spots corresponding to...

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Source

Identifiers

PMID: 9990481
DOI: 10.1097/00019606-199810000-00002

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CFTR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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