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Article

A colorimetric assay for phosphate to measure amplicon accumulation in polymerase chain reaction.

Analytical biochemistry - 1 Dec 1997

Gibson N J, Newton C R, Little S

Abstract excerpt

We describe a rapid colorimetric method for the detection of PCR products. Color generation is complete within 5 min of mixing reagents with a PCR. The method is simple and does not require affinity capture steps or special labeling to be carried out. The color development can be monitored by eye or a simple spectrophotometer can be used to read sample absorbance. We demonstrate the method by its use in the...

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Identifiers

PMID: 9398340
DOI: 10.1006/abio.1997.2324

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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