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Article

Intragenic recombinants of Plasmodium falciparum identified by in situ polymerase chain reaction.

Molecular and biochemical parasitology - 30 Jul 1999

Ranford-Cartwright L, Walliker D

Abstract excerpt

We report an in situ PCR technique for visualising amplified DNA of blood forms of Plasmodium falciparum on microscope slides by fluorescence microscopy. The method is used to assess the changes in frequency of different alleles of the MSP1 gene in cultures of the progeny of a cross. We show that parasites with a recombinant form of this protein possess an initial growth advantage before declining in numbers over...

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PMID: 10477172
DOI: 10.1016/s0166-6851(99)00069-9

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