Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Analysis of microsatellites by direct blotting electrophoresis and chemiluminescence detection.

Electrophoresis - 1 Oct 1995

Mekus F, Dörk T, Deufel T, Morral N, Tümmler B

Abstract excerpt

We describe a fast and reliable method for the nonradioactive analysis of microsatellites. For three dinucleotide repeats within the cystic fibrosis transmembrane conductance regulator (CFTR) gene, the separation of polymerase chain reaction (PCR) products generated with biotinylated primers on a direct blotting electrophoresis system and subsequent chemiluminescence detection is shown. In direct blotting...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Cystic Fibrosis Transmembrane Conductance Regulator
Share to DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CFTR gene

Share to Heterozygote

Share to Homozygote

Share to Microsatellite Repeats

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8586058
DOI: 10.1002/elps.11501601309

Genes

CFTR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Analysis of microsatellites by direct blotting electrophoresis and chemiluminescence detection.PMID 8586058Read ↗

A nonisotopic single-strand conformation polymorphism protocol using a direct blotting electrophoresis, a chemiluminescent detection system, and a multiplex approach.Semantic proximity Read ↗

Comprehensive and accurate mutation scanning of the CFTR gene by two-dimensional DNA electrophoresis.Semantic proximity Read ↗

Separation of PCR fragments by means of direct blotting electrophoresis.Semantic proximity Read ↗

High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.Semantic proximity Read ↗

Molecular epidemiology of cystic fibrosis mutations and haplotypes in southern Italy evaluated with an improved semiautomated robotic procedure.Semantic proximity Read ↗

Direct blotting electrophoresis for sequencing and genotyping hepatitis C virus.Semantic proximity Read ↗

PCR-SSCP: nonradioisotopic detection with biotinylated primers and streptavidin-alkaline phosphatase conjugate.Semantic proximity Read ↗

Mutation detection and typing of polymorphic loci through double-strand conformation analysis.Semantic proximity Read ↗

Simultaneous amplification and detection of specific DNA sequences.Semantic proximity Read ↗

Fluorescence-based oligonucleotide ligation assay for analysis of cystic fibrosis transmembrane conductance regulator gene mutations.Semantic proximity Read ↗

Simple non-radioactive detection of the CFTR mutation N1303K by artificial creation of a restriction site.Semantic proximity Read ↗

Simultaneous screening for 11 mutations in the cystic fibrosis transmembrane conductance regulator gene by multiplex amplification and reverse dot-blot.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.