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Sequence specific generation of a DNA panhardle permits PCR amplication of unknown flanking DNA

1 Jan 1992

Abstract excerpt

We present a novel method for the PCR amplification of unknown DNA that flanks a known segment directly from human genomic DNA. PCR requires that primer annealing sites be present on each end of the DNA segment that is to be amplified. In this method, known DNA is placed on the uncharacterized side of the sequence of interest via DNA polymerase mediated generation of a PCR template that is shaped like a pan with...

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PMID: 1371352
DOI: 10.1093/nar/20.3.595

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